Prolonged cell survival enhances peritoneal dissemination of gastric cancer cells
Bcl 2 and a Bcl 2 binding protein BAG 1 function in protection from apoptosis induced by a variety of stimuli. Deregulated expression of Bcl 2 leads to inhibition of apoptosis and is correlated with development of various cancers. Here, we provide evidence that prolonged cell survival introduced by overproduction of Bcl 2 or BAG 1 strongly enhances peritoneal dissemination of human gastric cancer MKN74 cells. Gene transfer mediated overexpression of Bcl 2 or BAG 1 led to prolonged cell survival of MKN74 cells against serum starved apoptosis and anoikis. When the viable transfectants were inoculated into the intraperitoneal cavity of BALB/c nude mice, the Bcl 2 expressing MKN74 cells and the BAG 1 expressing MKN74 cells exhibited strongly enhanced peritoneal dissemination in BALB/c nude mice and whole michael kors bag disseminated tumor weights were increase michael kors bag d by 4 fold and 3.3 fold, respectively, compared with the control transfectants. The enhanced peritoneal dissemination of MKN74 Bcl 2 and MKN74 BAG 1 transfectants correlated well with resistance to cell death induced by serum starvation and anoikis. However, the overexpression of Bcl 2 or BAG 1 caused no significant difference michael kors bag among the transfectants in cell growth rates, either in vitro or in vivo. Taken together, these studies demonstrate that michael kors bag resistance to apoptosis is a crucial factor for development of peritoneal dissemination of human gastric cancer cells.